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	Provedor de dados BJMBR (2) Autor Brunialti,M.K.C. (2) Mendes,M.E. (2) Salomão,R. (2) Fernandes,M.L. (1) Freudenberg,M. (1) Galanos,C. (1) Gomes,N.E. (1) Mais... Palavra-chave Cell surface receptors (1) Flagellin (1) IL-6 (1) LPS tolerance (1) Lipopolysaccharide (1) MALP-2 (1) Monocytes (1) Neutrophils (1) Nitric oxide (1) Phagocytosis (1) ROS (1) Reactive oxygen species (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2010 (2) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Human monocytes tolerant to LPS retain the ability to phagocytose bacteria and generate reactive oxygen species BJMBR Fernandes,M.L.; Mendes,M.E.; Brunialti,M.K.C.; Salomão,R.. Tolerance to lipopolysaccharide (LPS) occurs when animals or cells exposed to LPS become hyporesponsive to a subsequent challenge with LPS. This mechanism is believed to be involved in the down-regulation of cellular responses observed in septic patients. The aim of this investigation was to evaluate LPS-induced monocyte tolerance of healthy volunteers using whole blood. The detection of intracellular IL-6, bacterial phagocytosis and reactive oxygen species (ROS) was determined by flow cytometry, using anti-IL-6-PE, heat-killed Staphylococcus aureus stained with propidium iodide and 2',7'-dichlorofluorescein diacetate, respectively. Monocytes were gated in whole blood by combining FSC and SSC parameters and CD14-positive staining. The exposure to... Tipo: Info:eu-repo/semantics/article Palavras-chave: IL-6; ROS; Phagocytosis; MALP-2; Flagellin; LPS tolerance. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900008 Lipopolysaccharide-induced expression of cell surface receptors and cell activation of neutrophils and monocytes in whole human blood BJMBR Gomes,N.E.; Brunialti,M.K.C.; Mendes,M.E.; Freudenberg,M.; Galanos,C.; Salomão,R.. Lipopolysaccharide (LPS) activates neutrophils and monocytes, inducing a wide array of biological activities. LPS rough (R) and smooth (S) forms signal through Toll-like receptor 4 (TLR4), but differ in their requirement for CD14. Since the R-form LPS can interact with TLR4 independent of CD14 and the differential expression of CD14 on neutrophils and monocytes, we used the S-form LPS from Salmonella abortus equi and the R-form LPS from Salmonella minnesota mutants to evaluate LPS-induced activation of human neutrophils and monocytes in whole blood from healthy volunteers. Expression of cell surface receptors and reactive oxygen species (ROS) and nitric oxide (NO) generation were measured by flow cytometry in whole blood monocytes and neutrophils. The... Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipopolysaccharide; Neutrophils; Monocytes; Cell surface receptors; Reactive oxygen species; Nitric oxide. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900007 Registros recuperados: 2 Primeira ... 1 ... Última

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